NANOCURE Research

Cancer Research - Nanocure

Summary

Research in the NANOCURE (Nanotechnology-based Analysis Of Cancer through Undergraduate Research Experience) project involves the analysis of cancer therapeutics using a multidisciplinary approach that integrates biology, engineering, nanotechnology, optics, and more. Specifically, NANOCURE is interested in the potential for cesium (an alkali metal) to act as a cancer therapeutic using microfluidics and Raman spectroscopy.

The main purpose of this research project is to isolate single cells, specifically cancer cells. The cell is the fundamental unit of biological organisms and it is very important to study single cells in order to improve treatment methods. The mechanical device used to isolate single cells is a microfluidic device. A microfluidic device consists of small channels that can transport small amounts of fluids from one location to another. These small channels allow for controlling and manipulation of the fluids to provide the opportunity to isolate single cells. To verify changes in these single cells, Raman spectroscopy which uses laser light is used to provide a structural fingerprint.

Description of Research

One of the goals of biology is to understand how cells work. This goal requires various approaches for measurements on a single cell maintaining its structural integrity. Microfluidics can provide a solution for cell isolation and analysis. Microfluidic devices have emerged as distinct new structures that can precisely control the transport of fluids through channels at the micro and nano scale, and therefore have the ability to control and study small particles over time. This research uses microfluidic technology to manipulate movement of human colorectal carcinoma cells and entrap single cells.

Previous research at UW-Platteville demonstrated that cesium treatment eliminated colorectal tumors in mice without noticeable adverse effects. Complementary alternative medicine therapies based on the use of cesium chloride preparations for the treatment of cancer and radiation poisoning, have generated therapeutic interest; but oral or intravenous administration of cesium chloride (CsCl) to cancer patients as an alternative mode of cancer therapy have not been approved by the US Food and Drug Administration (FDA).  Our project investigates cesium’s suppressive effect human colorectal carcinoma cells in vitro.

The biochemical output of cancer cells is vastly different from normal cells, and in particular, cancer cells are highly acidic. A biocompatible microfluidic device capable of measuring the extracellular pH of human colorectal carcinoma cells was developed. The sensor’s electrodes consist of a silver chloride reference electrode and an iridium oxide-working electrode that provides real time voltage output in the mV range. Calibration was performed via linear regression of induced voltage as a function of pH, and the slope, or Nernstian value, provided relationship of 53mV/pH of the device. Current studies are being conducted to incorporate the pH sensor into a microfluidic device that isolates single cells. In addition, future research include Raman spectroscopy to provide quantitative information of the biochemical output of cells. Raman spectroscopy will be used to verify changes in biochemical composition that correspond to cellular pH changes with cesium treatment.

Academic Areas of Focus

Biology
This project is related to biology because it involves the use of microfluidics technology to study cell biology. Microfluidic devices enables studies of cell behavior from single- to multi-cellular organisms.

Nanotechnology ("nanotech")
This project operates in the design, fabrication and testing of micro- or nano-scale fluidic platforms. Some microfluidic devices rely on photolithography, a nanotechnology process, for fabrication.

Engineering
This project requires the understanding of the fundamentals of fluid mechanics for applications in the biomedical and engineering areas. For example, it is important to understand the different driving forces for controlling fluid flows in smaller scales to isolate single cells.

Senior Design a "Capstone Design Experience"
Senior Design is about students, working in small teams and tackling design challenges. Part of the success of the NANOCURE research is due to the work of several senior design teams.

  • Spring 2017 Mechanical senior design, designed and fabricated a microscope X-Y stage with precision motion to locate cancer cells in a microfluidics device.
    The team members: Jeremy Borg, Matt Dittmer, Adam Gagliardi, Tyler Huttenlocher, and Dan Wright.
     
  • Fall 2016 Mechanical senior design, designed and fabricated a microfluidic chip holder that allows one to control the temperature of the chip and provide electrical connections to the chip.
    The team members: Adriana Hernandez-Berrios, Austin Gaspar, Derek Thieme, Jacob Ahrenholtz, and Nicholas Devroy.
     
  • Spring 2016 Mechanical senior design, designed and fabricated a Raman Spectroscope to analyze cancer cells by obtaining vibrational information of molecules like chemical bonds.
    The team members: Bradley A Devroy, Victoria J Dittmann, Patrick T Meicher, Kevin M Norris, and Brian E Weiss.

Events

Wisconsin Science & Technology Symposium (WSTS 2017)
http://www.wisys.org/events/wsts

Pioneer Academic Center for Community Engagement (PACCE) Poster Day (2017) https://www.uwplatt.edu/pacce

UW System Undergraduate Research Symposium (2017)
https://www.uwsp.edu/oscar/Pages/symposium/default.aspx

Research in the Rotunda (2017)
https://www.wisconsin.edu/research-in-the-rotunda/

Application and Career Opportunities

There are many career opportunities for students with experience in microfluidics-based medical devices.

Microfluidics companies:
http://fluidicmems.com/list-of-microfluidics-lab-on-a-chip-and-biomems-companies/

Join our research group...

We are always looking for highly motivated students. If you are interested in a more in-depth research experience such as independent study credit, please contact Dr. Jorge Camacho if you are majoring in engineering (We welcome all engineering majors.) and you think that NANOCURE is the right project for you. Please contact Dr. Miranda Bader if you are majoring in biology and you think that NANOCURE is the right project for you.

EMS RESEARCH Nanocure Researchers

Meet the Researchers

Learn more about the Researchers
 

Faculty

Miranda Bader-Goodman

Miranda Bader-Goodman, PhD
Co-Principle Investigator
Biology

Jorge Camacho

Jorge Camacho, PhD
Co-Principle Investigator
Mechanical Engineering

Associated Faculty

Marilyn J. Tufte

Marilyn J. Tufte, PhD
Biology

Joseph Wu

Joseph Wu, PhD
Chemistry

Students

Kolt Bode

Kolt Bode
Lead Student Researcher
Major:Mechanical Engineering

Bridget White

Bridget White
Lead Student Researcher
Major:
Engineering Physics

John Dank

John Dank
Major:Biology

Carley Traverse

Carley Traverse
Major:Biology

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