Organic Chemistry 354 Exam - December 12, 1997

Dr. Sundin, University of Wisconsin-Platteville

6. (10%) Using standard abbreviations, give the basic structure of the polypeptide whose analytical hydrolysis data is shown below. Edman degradation gave Lys. Carboxypeptidase A analysis gave Ala.

Total Hydrolysis:

Ala = 2
Arg = 1
Cys = 1
Glu = 1
Leu = 1
Lys = 2
Phe = 1
Val = 1

Partial Hydrolysis:

Cys-Glu-Leu
Leu-Ala-Ala
Lys-Arg-Cys
Lys-Val-Phe