Organic Chemistry 354 Quiz - December 8, 2000
Dr. Sundin
- 1. A nonapeptide gave the following amino acids on complete hydrolysis:
- Ala, Asp, Glu, Gly, Leu, Lys, Phe, Tyr, Val
- Edman degradation gave Gly while carboxypeptidase A gave Val.
- Chymotrypsin hydrolysis (to the right of Phe, Try, Tyr) of the nonapeptide
gave several fragments of which only two were isolated in any quantity, a
pentapeptide and a tetrapeptide. Hydrolysis of the tetrapeptide gave
the three dipeptides below:
- Ala, Val; Asp, Ala; Asp, Phe
- Trypsin hydrolysis (to the right of Arg and Lys) of the nonapeptide
gave only two fragments, a pentapeptide and a tetrapeptide. Complete hydrolysis
of this tetrapeptide gave: Glu, Leu, Lys, Gly.
- Partial hydrolysis of this tetrapeptide gave a dipeptide which contained Glu, Gly.
- Deduce the primary structure of this nonapeptide.
Answer
- 2. What force or bond which is not responsible for the primary structure
of a protein is responsible for the secondary structure of a protein?
Answer
- 3. Speaking broadly of all enzymes, briefly list three different sub-functions
that portions of the enzyme might do as the enzyme performs its overall function.
Answer
- 4. The two dimensional structures of three amino acids are given below.
Draw the two dimensional structure of the tripeptide, Tyr-Glu-Leu.
Answer
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