Transferring volumes using the three-way safety bulb

Once you have drawn up the desired volume of solution and removed the drop hanging from the tip, record the initial volume in the pipet.

To transfer the solution into the desired vessel, press the empty valve (E) until the meniscus is at the mark corresponding to the appropriate volume. Touch the tip of the pipet to the wall of the receiving vessel to remove any liquid from the outside of the tip. Record the final volume in the pipet. The volume transferred is equal to the final pipet reading minus the initial pipet reading.